Recombinant Human Interleukin-4/IL-4 Protein (Active) Purity >95%

Recombinant Human Interleukin-4/IL-4 Protein (Active) Purity >95%

SKU #	PKSH033456
Expression Host	HEK293 Cells

Description

Synonyms	Interleukin,B-cell growth factor,BSF,IL4,BCGF-1,BCGF1,BSF-1,BSF1,IL-4,B-cell growth factor 1,B-cell IgG differentiation factor,B-cell stimulatory factor 1,Binetrakin,IGG1 induction factor,Interleukin-4,Lymphocyte stimulatory factor 1,Pitrakinra
Species	Human
Expression Host	HEK293 Cells
Sequence	His25-Ser153
Accession	P05112
Calculated Molecular Weight	15.0 kDa
Observed Molecular Weight	18 kDa
Tag	None
Bio-activity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.05 ng/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin	< 0.01 EU per g of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 m filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background
Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation; survival and gene expression. IL-4 is produced by mast cells; T cells; and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells; characterized by their cytokine-secretion profile that includes secretion of IL-4; IL-5; IL-6; IL-10; and IL-13; which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.