Rat INS(Insulin) ELISA Kit
| Detection Range | 6.25-400 pg/mL |
| Sensitivity | 3.75 pg/mL |
Product Details
Properties
| Assay type | Sandwich-ELISA |
| Format | 96T |
| Assay time | 3.5h |
| Detection range | 6.25-400 pg/mL |
| Sensitivity | 3.75 pg/mL |
| Sample type &Sample volume | serum, plasma and other biological fluids; 100L |
| Specificity | This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Application | This ELISA kit applies to the in vitro quantitative determination of Rat INS concentrations in serum, plasma and other biological fluids. |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat INS. You can calculate the concentration of Rat INS in the samples by comparing the OD of the samples to the standard curve.
Kit Components and Storage
An unopened kit can be stored at 2-8 for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
| Item | Specifications | Storage |
|---|
| Micro ELISA Plate(Dismountable) | 96T: 8 wells 12 strips
48T: 8 wells 6 strips | -20, 6 months |
| Reference Standard | 96T: 2 vials
48T: 1 vial |
| Concentrated Biotinylated Detection Ab (100) | 96T: 1 vial, 120 L
48T: 1 vial, 60 L |
| Concentrated HRP Conjugate (100) | 96T: 1 vial, 120 L
48T: 1 vial, 60 L | -20(Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL |
| HRP Conjugate Diluent | 1 vial, 14 mL |
| Concentrated Wash Buffer (25) | 1 vial, 30 mL |
| Substrate Reagent | 1 vial, 10 mL | 2-8(Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy |
| Certificate of Analysis | 1 copy |
Technical Data:
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat INS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat INS were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 19.26 | 40.93 | 195.61 | 17.58 | 47.64 | 176.39 |
| Standard deviation | 0.98 | 1.86 | 8.22 | 1.05 | 2.19 | 7.37 |
| CV (%) | 5.09 | 4.54 | 4.20 | 5.97 | 4.60 | 4.18 |
Recovery
The recovery of Rat INS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|
| Serum(n=8) | 86-99 | 94 |
| EDTA plasma (n=8) | 83-97 | 90 |
Target Information
| Database Links | SwissProt: P01322 P01323 |
| Synonyms | IDDM2, ILPR, IRDN, MODY10
|
| Research Area | Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction |
Assay Procedures
| 1. Add 100L standard or sample to the wells. Incubate for 90 min at 37C |
| 2. Discard the liquid, immediately add 100L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37C |
| 3. Aspirate and wash the plate for 3 times |
| 4. Add 100L HRP conjugate working solution. Incubate for 30 min at 37C. Aspirate and wash the plate for 5 times |
| 5. Add 90L Substrate Reagent. Incubate for 15 min at 37C |
| 6. Add 50L Stop Solution |
| 7. Read the plate at 450nm immediately. Calculation of the results |
